|
KMID : 0613820200300090804
|
|
Journal of Life Science
2020 Volume.30 No. 9 p.804 ~ p.812
|
|
|
Anti-inflammatory Activity of Antimicrobial Peptide Zophobacin 1 Derived from the Zophobas atratus
|
|
Shin Yong-Pyo
Lee Joon-Ha
Kim In-Woo
Seo Min-Chul
Kim Mi-Ae
Lee Hwa-Jeong
Baek Min-Hee
Kim Seong-Hyun
Hwang Jae-Sam
|
|
|
Abstract
|
|
|
|
The giant mealworm beetle, Zophobas atratus (Coleoptera: Tenebrionidae) has been used as a protein source for small pets and mammals. Recently, it was temporarily registered in the list of the Food Code. We previously performed an in silico analysis of the Zophobas atratus transcriptome to identify putative antimicrobial peptides and identified several antimicrobial peptide candidates. Among them, we assessed the antimicrobial and anti-inflammatory activities of zophobacin 1 that was selected bioinformatically based on its physicochemical properties against microorganisms and mouse macrophage Raw264.7 cells. Zophobacin 1 showed antimicrobial activities against microorganisms without inducing hemolysis and decreased the nitric oxide production of the lipopolysaccharide-induced Raw264.7 cells. Moreover, ELISA and Western blot analysis revealed that zophobacin 1 reduced expression levels of pro-inflammatory enzymes such as inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). We also investigated expression of pro-inflammatory cytokines (interleukin-6 and interleukin- 1β) production through quantitative real time-PCR and ELISA. Zophobacin 1 markedly reduced the expression level of cytokines through the regulation of mitogen-activated protein kinases (MAPKs) and nuclear factor kappa B (NF-κB) signaling. We confirmed that zophobacin 1 bound to bacterial cell membranes via a specific interaction with lipopolysaccharides. These data suggest that zophobacin 1 could be promising molecules for development as antimicrobial and anti-inflammatory therapeutic agents.
|
|
|
KEYWORD
|
|
|
Anti-inflammatory activity, antimicrobial activity, antimicrobial peptide, RNA sequencing, Zophobas atratus
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|